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KMID : 0903519950380010007
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1995 Volume.38 No. 1 p.7 ~ p.12
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Studies on the Development of Yeast Promoter for the Gene Expression
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Abstract
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The purpose of this study was the development of promoter for the lacZ´ gene. Two heterologous promoter Ⅰ and Ⅱ of lacZ´ gene were isolated from chromosomal DNA Bam HI fragment of yeast. The size of the promoter Ⅰ was estimated to be 2.5 kb and β-galacrosidase activity was 124.6 U/㎎ protein, and the size of the promoter Ⅱ was 4.0 kb and its β-galactosidase activity was 168.8 U/㎎ protein, respectively. The stability of the recombinant YEp plasmid in the transformant was from 52.7 to 67.4% at minimal medium. YIp plasmid was constructed from YEp plasmid, and expressed both in E. coli and yeast. The promoter Ⅰ aid Ⅱ iso-laced from yeast chromosomal DNA can be used for promoter of plasmid YEp and YIp.
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KEYWORD
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